dc.contributor.author | Jaén-Luchoro, Daniel | |
dc.contributor.author | Karlsson, Roger | |
dc.contributor.author | Busquets, Antonio | |
dc.contributor.author | Iglesias, Beatriz Piñeiro | |
dc.contributor.author | Karami, Nahid | |
dc.contributor.author | Marathe, Nachiket | |
dc.contributor.author | Moore, Edward RB | |
dc.date.accessioned | 2023-10-04T11:35:17Z | |
dc.date.available | 2023-10-04T11:35:17Z | |
dc.date.created | 2023-03-07T11:18:47Z | |
dc.date.issued | 2023 | |
dc.identifier.citation | Microbiology spectrum. 2023, 11 (1), . | |
dc.identifier.issn | 2165-0497 | |
dc.identifier.uri | https://hdl.handle.net/11250/3094124 | |
dc.description.abstract | Resistance to β-lactams is known to be multifactorial, although the underlying mechanisms are not well established. The aim of our study was to develop a system for assessing the phenotypic and proteomic responses of bacteria to antibiotic stress as a result of the loss of selected antimicrobial resistance genes. We applied homologous recombination to knock out plasmid-borne β-lactamase genes (blaOXA-1, blaTEM-1, and blaCTX-M15) in Escherichia coli CCUG 73778, generating knockout clone variants lacking the respective deleted β-lactamases. Quantitative proteomic analyses were performed on the knockout variants and the wild-type strain, using bottom-up liquid chromatography tandem mass spectrometry (LC-MS/MS), after exposure to different concentrations of cefadroxil. Loss of the blaCTX-M-15 gene had the greatest impact on the resulting protein expression dynamics, while losses of blaOXA-1 and blaTEM-1 affected fewer proteins’ expression levels. Proteins involved in antibiotic resistance, cell membrane integrity, stress, and gene expression and unknown function proteins exhibited differential expression. The present study provides a framework for studying protein expression in response to antibiotic exposure and identifying the genomic, proteomic, and phenotypic impacts of resistance gene loss. | |
dc.language.iso | eng | |
dc.title | Knockout of Targeted Plasmid-Borne β-Lactamase Genes in an Extended-Spectrum-β-Lactamase-Producing Escherichia coli Strain: Impact on Resistance and Proteomic Profile | |
dc.title.alternative | Knockout of Targeted Plasmid-Borne β-Lactamase Genes in an Extended-Spectrum-β-Lactamase-Producing Escherichia coli Strain: Impact on Resistance and Proteomic Profile | |
dc.type | Peer reviewed | |
dc.type | Journal article | |
dc.description.version | publishedVersion | |
dc.source.pagenumber | 22 | |
dc.source.volume | 11 | |
dc.source.journal | Microbiology spectrum | |
dc.source.issue | 1 | |
dc.identifier.doi | 10.1128/spectrum.03867-22 | |
dc.identifier.cristin | 2131884 | |
cristin.ispublished | true | |
cristin.fulltext | original | |
cristin.qualitycode | 1 | |