dc.contributor.author | Wischhusen, Pauline | |
dc.contributor.author | Heraud, Cécile | |
dc.contributor.author | Skjærven, Kaja Helvik | |
dc.contributor.author | Kaushik, Sadasivam J. | |
dc.contributor.author | Fauconneau, Benoit | |
dc.contributor.author | Prabhu, P. Antony Jesu | |
dc.contributor.author | Fontagné-Dicharry, Stéphanie | |
dc.date.accessioned | 2022-07-01T09:37:07Z | |
dc.date.available | 2022-07-01T09:37:07Z | |
dc.date.created | 2022-05-05T14:31:36Z | |
dc.date.issued | 2022 | |
dc.identifier.citation | British Journal of Nutrition. 2022, 127 (1), 23-34. | en_US |
dc.identifier.issn | 0007-1145 | |
dc.identifier.uri | https://hdl.handle.net/11250/3002024 | |
dc.description.abstract | This study evaluated how different forms of selenium (Se) supplementation into rainbow trout broodstock diets modified the one-carbon metabolism of the progeny after the beginning of exogenous feeding and followed by hypoxia challenge. The progeny of three groups of rainbow trout broodstock fed either a control diet (Se level: 0·3 µg/g) or a diet supplemented with inorganic sodium selenite (Se level: 0·6 µg/g) or organic hydroxy-selenomethionine (Se level: 0·6 µg/g) was cross-fed with diets of similar Se composition for 11 weeks. Offspring were sampled either before or after being subjected to an acute hypoxic stress (1·7 mg/l dissolved oxygen) for 30 min. In normoxic fry, parental Se supplementation allowed higher glutathione levels compared with fry originating from parents fed the control diet. Parental hydroxy-selenomethionine treatment also increased cysteine and cysteinyl–glycine concentrations in fry. Dietary Se supplementation decreased glutamate–cysteine ligase (cgl) mRNA levels. Hydroxy-selenomethionine feeding also lowered the levels of some essential free amino acids in muscle tissue. Supplementation of organic Se to parents and fry reduced betaine-homocysteine S-methyltransferase (bhmt) expression in fry. The hypoxic stress decreased whole-body homocysteine, cysteine, cysteinyl-glycine and glutathione levels. Together with the higher mRNA levels of cystathionine beta-synthase (cbs), a transsulphuration enzyme, this suggests that under hypoxia, glutathione synthesis through transsulphuration might have been impaired by depletion of a glutathione precursor. In stressed fry, S-adenosylmethionine levels were significantly decreased, but S-adenosylhomocysteine remained stable. Decreased bhmt and adenosylmethionine decarboxylase 1a (amd1a) mRNA levels in stressed fry suggest a nutritional programming by parental Se also on methionine metabolism of rainbow trout. | en_US |
dc.language.iso | eng | en_US |
dc.title | Long-term effect of parental selenium supplementation on the one-carbon metabolism in rainbow trout (Oncorhynchus mykiss) fry exposed to hypoxic stress | en_US |
dc.title.alternative | Long-term effect of parental selenium supplementation on the one-carbon metabolism in rainbow trout (Oncorhynchus mykiss) fry exposed to hypoxic stress | en_US |
dc.type | Peer reviewed | en_US |
dc.type | Journal article | en_US |
dc.description.version | publishedVersion | en_US |
dc.source.pagenumber | 23-34 | en_US |
dc.source.volume | 127 | en_US |
dc.source.journal | British Journal of Nutrition | en_US |
dc.source.issue | 1 | en_US |
dc.identifier.doi | 10.1017/S000711452100074X | |
dc.identifier.cristin | 2021828 | |
dc.relation.project | Havforskningsinstituttet: 15329 | en_US |
cristin.ispublished | true | |
cristin.fulltext | original | |
cristin.qualitycode | 2 | |