Detection of nodavirus in seawater from rearing facilities for Atlantic halibut Hippoglossus hippoglossus larvae

dc.contributor.author	Nerland, Audun H.
dc.contributor.author	Skår, Cecilie Kristin
dc.contributor.author	Eriksen, Tove Boge
dc.contributor.author	Bleie, Hogne
dc.date.accessioned	2007-07-25T10:26:04Z
dc.date.issued	2007-01-18
dc.identifier.issn	0177-5103
dc.identifier.uri	http://hdl.handle.net/11250/108504
dc.description	Journal home page: http://www.int-res.com/journals/dao/	en
dc.description.abstract	We used (1) ultracentrifugation followed by RT-PCR and (2) real-time RT-PCR to detect and quantify nodaviruses in seawater in which Atlantic halibut Hippoglossus hippoglossus larvae/fry had been held at rearing facilities. Evaluated against in vitro propagated viruses, the viral concentration corresponded to 1.6 × 104 TCID50 (50% tissue culture infectious dose) ml–1. Evaluated against in vitro transcribed RNA, the concentration was estimated at 2 × 107 virus particles ml–1 seawater.	en
dc.format.extent	455419 bytes
dc.format.mimetype	application/pdf
dc.language.iso	eng	en
dc.rights	Open access
dc.subject	halibut	en
dc.subject	kveite	en
dc.subject	nodavirus	en
dc.title	Detection of nodavirus in seawater from rearing facilities for Atlantic halibut Hippoglossus hippoglossus larvae	en
dc.type	Journal article	en
dc.type	Peer reviewed
dc.source.pagenumber	201-205	en
dc.source.volume	73	en
dc.source.journal	Diseases of Aquatic Organisms
dc.identifier.doi	10.3354/dao073201