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dc.contributor.authorSommerset, Ingunn
dc.contributor.authorNerland, Audun H.
dc.date.accessioned2008-05-06T14:35:32Z
dc.date.issued2004-03-10
dc.identifier.issn0177-5103
dc.identifier.issn1616-1580
dc.identifier.urihttp://hdl.handle.net/11250/108354
dc.description.abstractThe Nodaviridae are divided into the alphanodavirus genus, which infects insects, and the betanodavirus genus, which infects fishes. Betanodaviruses are the causative agent of viral encephalopathy and retinopathy (VER) in a number of cultivated marine fish species. The Nodaviridae are small non-enveloped RNA viruses that contain a genome consisting of 2 single-stranded positive-sense RNA segments: RNA1 (3.1 kb), which encodes the viral part of the RNA-dependent RNA polymerase (RdRp); and RNA2 (1.4 kb), which encodes the capsid protein. In addition to RNA I and RNA2, a subgenomic transcript of RNA1, RNA3, is present in infected cells. We have cloned and sequenced RNAI from the Atlantic halibut Hippoglossus hippoglossus nodavirus (AHNV), and for the first time, the sequence of a betanodaviral subgenomic RNA3 has been determined. AHNV RNAI was 3100 nucleotides in length and contained a main open reading frame encoding a polypeptide of 981 amino acids. Conservative motifs for RdRp were found in the deduced amino acid sequence. RNA3 was 371 nucleotides in length, and contained an open reading frame encoding a peptide of 75 amino acids corresponding to a hypothetical B2 protein, although sequence alignments with the alphdnodavirus B2 proteins showed only marginal similarities. AHNV RNA replication in the fish cell-line SSN-1 (derived from striped snakehead) was analysed by Northern blot analysis, which indicated that RNA3 was synthesised in large amounts (compared to RNA1) at an early point in time post-infection.en
dc.format.extent489591 bytes
dc.format.mimetypeapplication/pdf
dc.language.isoengen
dc.publisherInter Researchen
dc.subjectnodavirusen
dc.subjectkveiteen
dc.subjecthalibuten
dc.titleComplete sequence of RNA1 and subgenomic RNA3 of Atlantic halibut nodavirus (AHNV)en
dc.typeJournal articleen
dc.typePeer revieweden
dc.source.pagenumber117-125en
dc.source.volume58en
dc.source.journalDiseases of Aquatic Organismsen
dc.source.issue2-3en
dc.identifier.doihttp://dx.doi.org/10.3354/dao058117


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