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dc.contributor.authorChristie, K. E.
dc.contributor.authorHjeltnes, Brit K.
dc.contributor.authorUglenes, I.
dc.contributor.authorWinton, J. R.
dc.date.accessioned2007-06-20T07:46:51Z
dc.date.issued1993-04-29
dc.identifier.issn0177-5103
dc.identifier.urihttp://hdl.handle.net/11250/108324
dc.description.abstractPlasma was collected from Atlantic salmon Salmo salar with acute infectious salmon anaemia (ISA) and used to challenge Atlantic salmon parr by intraperitoneal injection. Treatment of plasma with the lipid solvent, chloroform, showed that the etiological agent of ISA contained essential lipids, probably as a viral envelope. Some infectivity remained following treatment with freon. Injection challenges using fractions from equilibrium density gradient centrifugation of plasma from fish with acute ISA revealed a band of infectivity in the range 1.184 to 1.262 g cm-3. The band was believed to contain both complete ISA-virus particles and infectious particles lacking a complete envelope, nucleocapsid or genome. Density gradient centrifugation of infectious plasma for enrichment of the putative ISA virus appeared to offer a suitable method for obtaining virus-specific nucleic acid for use in the construction of cDNA libraries.en
dc.format.extent327726 bytes
dc.format.mimetypeapplication/pdf
dc.language.isoengen
dc.subjectsalmonen
dc.subjectlaksen
dc.subjectinfectious salmon anaemia (ISA)en
dc.subjectinfeksiøs lakseanemi (ILA)
dc.subjectfish diseases
dc.subjectfiskesykdommer
dc.titleDetermination of buoyant density and sensitivity to chloroform and freon for the etiological agent of infectious salmon anaemiaen
dc.typeJournal articleen
dc.typePeer reviewed
dc.source.pagenumber225-228en
dc.source.volume15
dc.source.journalDiseases of Aquatic Organisms
dc.source.issue3
dc.identifier.doihttp://dx.doi.org/10.3354/dao015225


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