Rescue of germ cells in dnd crispant embryos opens the possibility to produce inherited sterility in Atlantic salmon
dc.contributor.author | Guralp, Hilal | |
dc.contributor.author | Skaftnesmo, Kai Ove | |
dc.contributor.author | Kjærner-Semb, Erik Nordtorp | |
dc.contributor.author | Straume, Anne Hege | |
dc.contributor.author | Kleppe, Lene | |
dc.contributor.author | Schulz, Rüdiger W. | |
dc.contributor.author | Edvardsen, Rolf Brudvik | |
dc.contributor.author | Wargelius, Anna | |
dc.date.accessioned | 2020-12-15T13:32:01Z | |
dc.date.available | 2020-12-15T13:32:01Z | |
dc.date.created | 2020-11-25T11:42:29Z | |
dc.date.issued | 2020 | |
dc.identifier.citation | Scientific Reports. 2020, 10 . | en_US |
dc.identifier.issn | 2045-2322 | |
dc.identifier.uri | https://hdl.handle.net/11250/2719586 | |
dc.description.abstract | Genetic introgression of escaped farmed Atlantic salmon (Salmo salar) into wild populations is a major environmental concern for the salmon aquaculture industry. Using sterile fish in commercial aquaculture operations is, therefore, a sustainable strategy for bio-containment. So far, the only commercially used methodology for producing sterile fish is triploidization. However, triploid fish are less robust. A novel approach in which to achieve sterility is to produce germ cell-free salmon, which can be accomplished by knocking out the dead-end (dnd) gene using CRISPR-Cas9. The lack of germ cells in the resulting dnd crispants, thus, prevents reproduction and inhibits subsequent large-scale production of sterile fish. Here, we report a rescue approach for producing germ cells in Atlantic salmon dnd crispants. To achieve this, we co-injected the wild-type (wt) variant of salmon dnd mRNA together with CRISPR-Cas9 constructs targeting dnd into 1-cell stage embryos. We found that rescued one-year-old fish contained germ cells, type A spermatogonia in males and previtellogenic primary oocytes in females. The method presented here opens a possibility for large-scale production of germ-cell free Atlantic salmon offspring through the genetically sterile broodstock which can pass the sterility trait on the next generation. | en_US |
dc.language.iso | eng | en_US |
dc.title | Rescue of germ cells in dnd crispant embryos opens the possibility to produce inherited sterility in Atlantic salmon | en_US |
dc.type | Peer reviewed | en_US |
dc.type | Journal article | en_US |
dc.description.version | publishedVersion | en_US |
dc.source.pagenumber | 12 | en_US |
dc.source.volume | 10 | en_US |
dc.source.journal | Scientific Reports | en_US |
dc.identifier.doi | 10.1038/s41598-020-74876-2 | |
dc.identifier.cristin | 1852155 | |
cristin.ispublished | true | |
cristin.fulltext | original | |
cristin.qualitycode | 1 |
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