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dc.contributor.authorAranguren Abadía, Libe
dc.contributor.authorDonald, Carey
dc.contributor.authorEilertsen, Mariann
dc.contributor.authorGharbi, Naouel
dc.contributor.authorTronci, Valentina
dc.contributor.authorSørhus, Elin
dc.contributor.authorMayer, Philipp
dc.contributor.authorNilsen, Tom Ole
dc.contributor.authorMeier, Sonnich
dc.contributor.authorGoksøyr, Anders
dc.contributor.authorKarlsen, Odd Andre
dc.date.accessioned2020-10-15T11:46:56Z
dc.date.available2020-10-15T11:46:56Z
dc.date.created2020-07-24T14:46:28Z
dc.date.issued2020
dc.identifier.citationAquatic Toxicology. 2020, 226 .en_US
dc.identifier.issn0166-445X
dc.identifier.urihttps://hdl.handle.net/11250/2683073
dc.description.abstractThe aryl hydrocarbon receptor (Ahr) is a ligand-activated transcription factor that mediates the toxicity of dioxins and dioxin-like compounds (DLCs) in vertebrates. Two clades of the Ahr family exist in teleosts (Ahr1 and Ahr2), and it has been demonstrated that Ahr2 is the main protein involved in mediating the toxicity of dioxins and DLCs in most teleost species. Recently, we characterized the Atlantic cod (Gadus morhua) Ahr1a and Ahr2a receptors. To further explore a possible subfunction partitioning of Ahr1a and Ahr2a in Atlantic cod we have mapped the expression and localization of ahr1a and ahr2a in early developmental stages. Atlantic cod embryos were continuously exposed in a passive-dosing exposure system to the Ahr agonist, benzo[a]pyrene (B[a]P), from five days post fertilization (dpf) until three days post hatching (dph). Expression of ahr1a, ahr2a, and the Ahr-target genes, cyp1a and ahrrb, was assessed in embryos (8 dpf and 10 dpf) and larvae (3 dph) with quantitative real-time PCR analyses (qPCR), while in situ hybridization was used to assess the localization of expression of ahr1a, ahr2a and cyp1a. Quantitative measurements showed an increased cyp1a expression in B[a]P-exposed samples at all sampling points, and for ahr2a at 10 dpf, confirming the activation of the Ahr-signalling pathway. Furthermore, B[a]P strongly induced ahr2a and cyp1a expression in the cardiovascular system and skin, respectively, of embryos and larvae. Induced expression of both ahr2a and cyp1a was also revealed in the liver of B[a]P-exposed larvae. Our results suggest that Ahr2a is the major subtype involved in mediating responses to B[a]P in early developmental stages of Atlantic cod, which involves transcriptional regulation of biotransformation genes, such as cyp1a. The focused expression of ahr1a in the eye of embryos and larvae, and the presence of ahr2a transcripts in the jaws and fin nodes, further indicate evolved specialized roles of the two Ahrs in ontogenesis.en_US
dc.language.isoengen_US
dc.titleExpression and localization of the aryl hydrocarbon receptors and cytochrome P450 1A during early development of Atlantic cod (Gadus morhua)en_US
dc.typePeer revieweden_US
dc.typeJournal articleen_US
dc.description.versionpublishedVersionen_US
dc.source.pagenumber10en_US
dc.source.volume226en_US
dc.source.journalAquatic Toxicologyen_US
dc.identifier.doi10.1016/j.aquatox.2020.105558
dc.identifier.cristin1820448
dc.relation.projectNorges forskningsråd: 244564en_US
dc.relation.projectNorges forskningsråd: 254894en_US
dc.relation.projectNorges forskningsråd: 248840en_US
dc.relation.projectNorges forskningsråd: 267820en_US
cristin.ispublishedtrue
cristin.fulltextoriginal
cristin.qualitycode2


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