• norsk
    • English
  • English 
    • norsk
    • English
  • Login
View Item 
  •   Home
  • Havforskningsinstituttet
  • Publikasjoner fra CRIStin
  • View Item
  •   Home
  • Havforskningsinstituttet
  • Publikasjoner fra CRIStin
  • View Item
JavaScript is disabled for your browser. Some features of this site may not work without it.

Chlorpyrifos-induced dysfunction of lipid metabolism is not restored by supplementation of polyunsaturated fatty acids EPA and ARA in Atlantic salmon liver cells

Olsvik, Pål Asgeir; Hammer, SK; Sanden, Monica; Søfteland, Liv Ingeborg Rosvoll
Journal article, Peer reviewed
Published version
Thumbnail
View/Open
1-s2.0-S0887233319305041-main.pdf (3.311Mb)
URI
http://hdl.handle.net/11250/2639988
Date
2019
Metadata
Show full item record
Collections
  • Articles [2369]
  • Publikasjoner fra CRIStin [2142]
Original version
Toxicology in Vitro. 2019, 61 1-13.   10.1016/j.tiv.2019.104655
Abstract
Exposure to contaminants can lead to accumulation of lipids in the liver. This study aimed to examine whether eicosapentaenoic acid (EPA) and arachidonic acid (ARA) supplementation can protect fish cells against the negative impact of chlorpyrifos (CPF). Atlantic salmon hepatocytes were exposed to either 100 μM CPF, 200 μM EPA, 200 μM ARA, or combinations of these for 48 h, and endpoints included lipid droplet formation, gene expression, and global metabolomic analysis. The results showed that polyunsaturated fatty acid (PUFA) supplementation modified the cell lipid composition, reduced uptake of CPF and increased the cellular number and size of lipid droplets. CPF exposure induced the transcription of ppara and fabp3, and reduced the levels of several PUFAs, and lead to accumulation of monoacylglycerols (MAGs) in the cells. Supplementation of EPA or ARA did not prevent CPF-induced accumulation of MAGs and only to a limited degree rescued the response on other lipids. CPF exposure further reduced energy metabolism, a response partly restored by PUFA supplementation. Reduced levels of glutathione indicated oxidative stress; an effect not ameliorated by the PUFAs. Altogether, this study shows that PUFA supplementation only modestly protects Atlantic salmon hepatocytes against the negative impact of CPF.
Journal
Toxicology in Vitro

Contact Us | Send Feedback

Privacy policy
DSpace software copyright © 2002-2019  DuraSpace

Service from  Unit
 

 

Browse

ArchiveCommunities & CollectionsBy Issue DateAuthorsTitlesSubjectsDocument TypesJournalsThis CollectionBy Issue DateAuthorsTitlesSubjectsDocument TypesJournals

My Account

Login

Statistics

View Usage Statistics

Contact Us | Send Feedback

Privacy policy
DSpace software copyright © 2002-2019  DuraSpace

Service from  Unit