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dc.contributor.authorCoucheron, Dag H
dc.contributor.authorWojewodzic, Marcin
dc.contributor.authorBøhn, Thomas
dc.date.accessioned2020-01-10T09:44:17Z
dc.date.available2020-01-10T09:44:17Z
dc.date.created2019-12-08T20:36:15Z
dc.date.issued2019
dc.identifier.citationScientific Reports. 2019, 9 1-15.nb_NO
dc.identifier.issn2045-2322
dc.identifier.urihttp://hdl.handle.net/11250/2635630
dc.description.abstractMicroRNAs (miRNAs) are small non-coding RNAs that function in RNA silencing and post-transcriptional regulation of gene expression in most organisms. The water flea, Daphnia magna is a key model to study phenotypic, physiological and genomic responses to environmental cues and miRNAs can potentially mediate these responses. By using deep sequencing, genome mapping and manual curations, we have characterised the miRNAome of D. magna. We identified 66 conserved miRNAs and 13 novel miRNAs; all of these were found in the three studied life stages of D. magna (juveniles, subadults, adults), but with variation in expression levels between stages. Forty-one of the miRNAs were clustered into 13 genome clusters also present in the D. pulex genome. Most miRNAs contained sequence variants (isomiRs). The highest expressed isomiRs were 3′ template variants with one nucleotide deletion or 3′ non-template variants with addition of A or U at the 3′ end. We also identified offset RNAs (moRs) and loop RNAs (loRs). Our work extends the base for further work on all species (miRNA, isomiRs, moRNAs, loRNAs) of the miRNAome of Daphnia as biomarkers in response to chemical substances and environment cues, and underline age dependency.nb_NO
dc.language.isoengnb_NO
dc.titleMicroRNAs in Daphnia magna identified and characterized by deep sequencing, genome mapping and manual curationnb_NO
dc.typeJournal articlenb_NO
dc.typePeer reviewednb_NO
dc.description.versionpublishedVersionnb_NO
dc.source.pagenumber1-15nb_NO
dc.source.volume9nb_NO
dc.source.journalScientific Reportsnb_NO
dc.identifier.doi10.1038/s41598-019-52387-z
dc.identifier.cristin1757971
dc.relation.projectNorges forskningsråd: 238820nb_NO
dc.relation.projectEC/FP7/629892nb_NO
cristin.unitcode7431,17,0,0
cristin.unitnameSykdom og smittespredning
cristin.ispublishedtrue
cristin.fulltextoriginal
cristin.qualitycode1


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