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dc.contributor.authorWargelius, Anna
dc.contributor.authorLeininger, Sven Dirk
dc.contributor.authorSkaftnesmo, Kai Ove
dc.contributor.authorKleppe, Lene
dc.contributor.authorAndersson, Eva
dc.contributor.authorTaranger, Geir Lasse
dc.contributor.authorSchulz, Rüdiger W.
dc.contributor.authorEdvardsen, Rolf
dc.date.accessioned2016-08-12T12:16:21Z
dc.date.accessioned2016-08-16T11:45:50Z
dc.date.available2016-08-12T12:16:21Z
dc.date.available2016-08-16T11:45:50Z
dc.date.issued2016-02-18
dc.identifier.citationScientific Reports 2016, 6nb_NO
dc.identifier.issn2045-2322
dc.identifier.urihttp://hdl.handle.net/11250/2399377
dc.description-nb_NO
dc.description.abstractIntrogression of farmed salmon escapees into wild stocks is a major threat to the genetic integrity of wild populations. Using germ cell-free fish in aquaculture may mitigate this problem. Our study investigated whether it is possible to produce germ cell-free salmon in F0 by using CRISPR-Cas9 to knock out dnd, a factor required for germ cell survival in vertebrates. To avoid studying mosaic animals, sgRNA targeting alb was simultaneously used as a visual tracer since the phenotype of alb KO is complete loss of pigmentation. Induced mutations for the tracer (alb) and the target (dnd) genes were highly correlated and produced germ cell-less fish lacking pigmentation, underlining the suitability of alb KO to serve as tracer for targeted double allelic mutations in F0 animals in species with prohibitively long generation times. This is also the first report describing dnd knockout in any fish species. Analyzing gene expression and histology of dnd KO fish revealed that sex differentiation of the somatic compartment does not depend on the presence of germ cells. However, the organization of the ovarian somatic compartment seems compromised in mutant fish.nb_NO
dc.language.isoengnb_NO
dc.publisherNature Publishing Groupnb_NO
dc.rightsNavngivelse 3.0 Norge*
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/no/*
dc.titleDnd knockout ablates germ cells and demonstrates germ cell independent sex differentiation in Atlantic salmonnb_NO
dc.typeJournal articlenb_NO
dc.typePeer reviewednb_NO
dc.date.updated2016-08-12T12:16:21Z
dc.subject.nsiVDP::Matematikk og naturvitenskap: 400::Basale biofag: 470::Genetikk og genomikk: 474nb_NO
dc.subject.nsiVDP::Mathematics and natural scienses: 400::Basic biosciences: 470::Genetics and genomics: 474nb_NO
dc.source.pagenumber8 s.nb_NO
dc.source.volume6nb_NO
dc.source.journalScientific Reportsnb_NO
dc.identifier.doi10.1038/srep21284
dc.identifier.cristin1366420
dc.relation.projectNorges forskningsråd: 221648nb_NO


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