Blar i Brage IMR på forfatter "Straume, Anne Hege"
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In vivo CRISPR/LbCas12a-mediated knock-in and knock-out in Atlantic salmon (Salmo salar L.)
Raudstein, Mari Torsdotter; Kjærner-Semb, Erik Nordtorp; Barvik, Morten; Broll, Silje; Straume, Anne Hege; Edvardsen, Rolf Brudvik (Peer reviewed; Journal article, 2023)Genome editing using the CRISPR/Cas system offers the potential to enhance current breeding programs and introduce desirable genetic traits, including disease resistance, in salmon aquaculture. Several nucleases are available ... -
Indel locations are determined by template polarity in highly efficient in vivo CRISPR/Cas9- mediated HDR in Atlantic salmon
Straume, Anne Hege; Kjærner-Semb, Erik Nordtorp; Skaftnesmo, Kai Ove; guralp, hilal; Kleppe, Lene; Wargelius, Anna; Edvardsen, Rolf Brudvik (Peer reviewed; Journal article, 2020)Precise gene editing such as CRISPR/Cas9-mediated homology directed repair (HDR) can increase our understanding of gene function and improve traits of importance for aquaculture. This fine-tuned technology has not been ... -
Rescue of germ cells in dnd crispant embryos opens the possibility to produce inherited sterility in Atlantic salmon
Guralp, Hilal; Skaftnesmo, Kai Ove; Kjærner-Semb, Erik Nordtorp; Straume, Anne Hege; Kleppe, Lene; Schulz, Rüdiger W.; Edvardsen, Rolf Brudvik; Wargelius, Anna (Peer reviewed; Journal article, 2020)Genetic introgression of escaped farmed Atlantic salmon (Salmo salar) into wild populations is a major environmental concern for the salmon aquaculture industry. Using sterile fish in commercial aquaculture operations is, ... -
Single nucleotide replacement in the Atlantic salmon genome using CRISPR/Cas9 and asymmetrical oligonucleotide donors
Straume, Anne Hege; Kjærner-Semb, Erik Nordtorp; Skaftnesmo, Kai Ove; Guralp, Hilal; Lillico, Simon; Wargelius, Anna; Edvardsen, Rolf Brudvik (Peer reviewed; Journal article, 2021)New breeding technologies (NBT) using CRISPR/Cas9-induced homology directed repair (HDR) has the potential to expedite genetic improvement in aquaculture. The long generation time in Atlantic salmon makes breeding an ...