Accumulation and metabolism of phenanthrene in rainbow trout (Salmo gaidneri)
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http://hdl.handle.net/11250/103377Utgivelsesdato
1979Metadata
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This report is not to be quoted without prior consultation with the General Secretary.Sammendrag
Phenanthrene labelled with carbon-14 was administered to rainbow trout, Salmo
gaidneri, in dry food and the radioactivity in the different tissues were
measured at intervals using a Packard Tri-Carb scintillation-counter. Carbon- ,
14 was rapidly accumulated in the liver and thereafter high concentrations
was found in the gall bladder. Two weeks later most of the carbon-14 had disappeared.
The metabolites of phenanthrene were isolated from the gall bladder and
intestine 48 hours after the administration of 25 mg. The analyses of the
TMS-derivatives of the metabolites were performed using a computerized gas
chromatograph mass spectrometer. Quantitative studies were carried out using
selected ion monitoring. A total of eight metabolites were isolated and
identified. The main metabolite in gall bladder was 1.2-dihydro-1.2-dihydroxyphenanthrene,
which constituted 93% of the total metabolic products. The
fraction of free metabolites was 75 and 8% in gall bladder and intestine,
respectively.