Blar i Brage IMR på forfatter "Skaftnesmo, Kai Ove"
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Dnd knockout ablates germ cells and demonstrates germ cell independent sex differentiation in Atlantic salmon
Wargelius, Anna; Leininger, Sven Dirk; Skaftnesmo, Kai Ove; Kleppe, Lene; Andersson, Eva; Taranger, Geir Lasse; Schulz, Rüdiger W.; Edvardsen, Rolf (Journal article; Peer reviewed, 2016-02-18)Introgression of farmed salmon escapees into wild stocks is a major threat to the genetic integrity of wild populations. Using germ cell-free fish in aquaculture may mitigate this problem. Our study investigated whether ... -
Full production cycle performance of gene-edited, sterile Atlantic salmon - growth, smoltification, welfare indicators and fillet composition
Kleppe, Lene; Fjelldal, Per Gunnar; Andersson, Eva; Hansen, Tom Johnny; Sanden, Monica; Bruvik, Aina; Skaftnesmo, Kai Ove; Furmanek, Tomasz; Kjærner-Semb, Erik Nordtorp; Crespo, Diego; Flavell, Simon; Pedersen, Audun Østby; Vogelsang, Petra; Torsvik, Anja; Kvestad, Karen Anita; Olausson, Sara; Norberg, Birgitta; Schulz, Rüdiger W.; Bogerd, J.; Santi, N.; Edvardsen, Rolf Brudvik; Wargelius, Anna (Peer reviewed; Journal article, 2022)Using germ cell-free (GCF), sterile, dnd-knockout salmon for farming could solve the problems associated with precocious maturation and genetic introgression of farmed breeds into wild populations. However, prior to using ... -
Indel locations are determined by template polarity in highly efficient in vivo CRISPR/Cas9- mediated HDR in Atlantic salmon
Straume, Anne Hege; Kjærner-Semb, Erik Nordtorp; Skaftnesmo, Kai Ove; guralp, hilal; Kleppe, Lene; Wargelius, Anna; Edvardsen, Rolf Brudvik (Peer reviewed; Journal article, 2020)Precise gene editing such as CRISPR/Cas9-mediated homology directed repair (HDR) can increase our understanding of gene function and improve traits of importance for aquaculture. This fine-tuned technology has not been ... -
Inhibition of mitochondrial respiration prevents BRAF-mutant melanoma brain metastasis
Sundstrøm, Terje; Prestegarden, Lars; Azuaje, Francisco; Aasen, Synnøve Nymark; Røsland, Gro Vatne; Varughese, Jobin K.; Bahador, Marzieh; Bernatz, Simon; Braun, Yannick; Harter, Patrick N.; Skaftnesmo, Kai Ove; Ingham, Elizabeth S.; Mahakian, Lisa M.; Tam, Sarah; Tepper, Clifford G.; Petersen, Kjell; Ferrara, Katherine W.; Tronstad, Karl Johan; Lund-Johansen, Morten; Beschorner, Rudi; Bjerkvig, Rolf; Thorsen, Frits (Journal article; Peer reviewed, 2019)Melanoma patients carry a high risk of developing brain metastases, and improvements in survival are still measured in weeks or months. Durable disease control within the brain is impeded by poor drug penetration across ... -
Insulin-like 3 affects zebrafish spermatogenic cells directly and via Sertoli cells
Crespo, Diego; Assis, Luiz H. C.; Zhang, Yu Ting; Safian, Diego; Furmanek, Tomasz; Skaftnesmo, Kai Ove; Norberg, Birgitta; Ge, Wei; Choi, Yung-Ching; den Broeder, Marjo J.; Legler, Juliette; Bogerd, Jan; Schulz, Rüdiger W (Peer reviewed; Journal article, 2021)Pituitary hormones can use local signaling molecules to regulate target tissue functions. In adult zebrafish testes, follicle-stimulating hormone (Fsh) strongly increases the production of insulin-like 3 (Insl3), a Leydig ... -
Integrative testis transcriptome analysis reveals differentially expressed miRNAs and their mRNA targets during early puberty in Atlantic salmon
Skaftnesmo, Kai Ove; Edvardsen, Rolf; Furmanek, Tomasz; Crespo, D.; Andersson, Eva; Kleppe, Lene; Taranger, Geir Lasse; Bogerd, Jan; Schulz, Rüdiger W.; Wargelius, Anna (Peer reviewed; Journal article, 2017) -
Loss of stra8 Increases Germ Cell Apoptosis but Is Still Compatible With Sperm Production in Atlantic Salmon (Salmo salar)
Skaftnesmo, Kai Ove; Crespo, Diego; Kleppe, Lene; Andersson, Eva; Edvardsen, Rolf Brudvik; Norberg, Birgitta; Fjelldal, Per Gunnar; Hansen, Tom Johnny; Schulz, Rüdiger W.; Wargelius, Anna (Peer reviewed; Journal article, 2021)Entering meiosis strictly depends on stimulated by retinoic acid 8 (Stra8) gene function in mammals. This gene is missing in a number of fish species, including medaka and zebrafish, but is present in the majority of fishes, ... -
Microbial communities associated with the parasitic copepod Lepeophtheirus salmonis.
Dalvin, Sussie; Skaftnesmo, Kai Ove; Malde, Ketil; Glover, Kevin; Leong, Jong S; Koop, Ben F.; Edvardsen, Rolf Brudvik; Sandlund, Nina (Peer reviewed; Journal article, 2019)Lepeophtheirus salmonis is a naturally occurring marine parasite of salmonid fishes in the Northern hemisphere, and a major problem in salmonid aquaculture. In addition to the direct effects on host fish, L. salmonis may ... -
Pituitary Gonadotropin Gene Expression During Induced Onset of Postsmolt Maturation in Male Atlantic Salmon: In Vivo and Tissue Culture Studies
Crespo, Diego; Skaftnesmo, Kai Ove; Kjærner-Semb, Erik Nordtorp; Yilmaz, Ozlem; Norberg, Birgitta; Olausson, Sara; Vogelsang, Petra; Bogerd, Jan; Kleppe, Lene; Edvardsen, Rolf Brudvik; Andersson, Eva; Wargelius, Anna; Hansen, Tom Johnny; Fjelldal, Per Gunnar; Schulz, Rüdiger W. (Peer reviewed; Journal article, 2022)Precocious male maturation causes reduced welfare and increased production costs in Atlantic salmon (Salmo salar) aquaculture. The pituitary produces and releases follicle-stimulating hormone (Fsh), the gonadotropin ... -
The Piwil1 N domain is required for germ cell survival in Atlantic salmon
Almeida, Fernanda Loureiro; Skaftnesmo, Kai Ove; Andersson, Eva; Kleppe, Lene; Edvardsen, Rolf Brudvik; Norberg, Birgitta; Fjelldal, Per Gunnar; Hansen, Tom Johnny; Schulz, Rüdiger W.; Wargelius, Anna (Peer reviewed; Journal article, 2022)Genetic introgression of farmed salmon into wild populations can damage the genetic integrity of wild stocks and is therefore considered as an environmental threat. One possible solution is to induce sterility in farmed ... -
Rescue of germ cells in dnd crispant embryos opens the possibility to produce inherited sterility in Atlantic salmon
Guralp, Hilal; Skaftnesmo, Kai Ove; Kjærner-Semb, Erik Nordtorp; Straume, Anne Hege; Kleppe, Lene; Schulz, Rüdiger W.; Edvardsen, Rolf Brudvik; Wargelius, Anna (Peer reviewed; Journal article, 2020)Genetic introgression of escaped farmed Atlantic salmon (Salmo salar) into wild populations is a major environmental concern for the salmon aquaculture industry. Using sterile fish in commercial aquaculture operations is, ... -
Sex steroid production associated with puberty is absent in germ cellfree salmon
Kleppe, Lene; Andersson, Eva; Skaftnesmo, Kai Ove; Edvardsen, Rolf; Fjelldal, Per Gunnar; Norberg, Birgitta; Bogerd, Jan; Schulz, Rüdiger W.; Wargelius, Anna (Peer reviewed; Journal article, 2017) -
Single nucleotide replacement in the Atlantic salmon genome using CRISPR/Cas9 and asymmetrical oligonucleotide donors
Straume, Anne Hege; Kjærner-Semb, Erik Nordtorp; Skaftnesmo, Kai Ove; Guralp, Hilal; Lillico, Simon; Wargelius, Anna; Edvardsen, Rolf Brudvik (Peer reviewed; Journal article, 2021)New breeding technologies (NBT) using CRISPR/Cas9-induced homology directed repair (HDR) has the potential to expedite genetic improvement in aquaculture. The long generation time in Atlantic salmon makes breeding an ... -
The surveillance and control programme for bonamiosis and marteiliosis in European flat oysters, Ostrea edulis, and blue mussels, Mytilus sp. in Norway in 2022
Mortensen, Stein; Skår, Cecilie Kristin; Bøgwald, Mats; Ghebretnsae, Dawit Berhe; Skaftnesmo, Kai Ove; Jelmert, Anders (Rapport fra havforskningen;2023 - 25, Research report, 2023)The surveillance programme is carried out by the Institute of Marine Research according to a contract with the Norwegian Food Safety Authority. In 2022, samples were collected from an area in Agder where flat oysters and ... -
Targeted Mutagenesis in Atlantic Salmon (Salmo salar L.) Using the CRISPR/Cas9 System Induces Complete Knockout Individuals in the F0 Generation
Edvardsen, Rolf B.; Leininger, Svein; Kleppe, Lene; Skaftnesmo, Kai Ove; Wargelius, Anna (Journal article; Peer reviewed, 2014-09-25)Understanding the biological function behind key proteins is of great concern in Atlantic salmon, both due to a high commercial importance and an interesting life history. Until recently, functional studies in salmonids ... -
Transcriptomic analysis of dead end knockout testis reveals germ cell and gonadal somatic factors in Atlantic salmon
Kleppe, Lene; Edvardsen, Rolf Brudvik; Furmanek, Tomasz; Andersson, Eva; Skaftnesmo, Kai Ove; Thyri Segafredo, Frida; Wargelius, Anna Sofie Troedsson (Peer reviewed; Journal article, 2020)Sustainability challenges are currently hampering an increase in salmon production. Using sterile salmon can solve problems with precocious puberty and genetic introgression from farmed escapees to wild populations. Recently ... -
Vgll3 and the Hippo pathway are regulated in Sertoli cells upon entry and during puberty in Atlantic salmon testis
Kjærner-Semb, Erik Nordtorp; Ayllon, Fernando; Kleppe, Lene; Sørhus, Elin; Skaftnesmo, Kai Ove; Furmanek, Tomasz; Segafredo, Frida Thyri; Thorsen, Anders; Fjelldal, Per Gunnar; Hansen, Tom Johnny; Taranger, Geir Lasse; Andersson, Eva; Schulz, Rüdiger W.; Wargelius, Anna; Edvardsen, Rolf (Journal article; Peer reviewed, 2018)Vgll3 is linked to age at maturity in Atlantic salmon (Salmo salar). However, the molecular mechanisms involving Vgll3 in controlling timing of puberty as well as relevant tissue and cell types are currently unknown. Vgll3 ...