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dc.contributor.authorGrevskott, Didrik Hjertaker
dc.contributor.authorZamanzad Ghavidel, Fatemeh
dc.contributor.authorSvanevik, Cecilie Smith
dc.contributor.authorMarathe, Nachiket
dc.date.accessioned2021-10-04T09:01:13Z
dc.date.available2021-10-04T09:01:13Z
dc.date.created2021-09-30T15:10:40Z
dc.date.issued2021
dc.identifier.citationEcotoxicology and Environmental Safety. 2021, .en_US
dc.identifier.issn0147-6513
dc.identifier.urihttps://hdl.handle.net/11250/2787398
dc.description.abstractThe aim of this study was to examine antibiotic resistance profiles and diversity of β-lactamases in Escherichia coli present within the population and the potential spread of resistant E. coli into the receiving environment using city-scale sewage surveillance. In E. coli isolates from ECC plates without antibiotics from ten influent samples (n = 300), highest resistance was observed against ampicillin (16.6%), sulfamethoxazole (9.7%) and trimethoprim (9.0%), while in effluent samples (n = 262) it was against sulfamethoxazole (11.8%), ampicillin (11.5%) and tetracycline (8.8%). All isolates (n = 123) obtained on cefotaxime-containing plates were multidrug-resistant. Several clinically important antibiotic resistance genes (ARGs) were detected in 46 E. coli isolates subjected to whole-genome sequencing, including carbapenemases like NDM-6, VIM-1 and OXA-48-variant, as well as tigecycline resistance gene tet(X4). CTX-M-15 was the most prevalent (42.9%) extended-spectrum β-lactamase among cefotaxime-resistant isolates, followed by CTX-M-27 (31.4%) and CTX-M-14 (17.1%), resembling clinical prevalence in Norway. Most of the sequenced isolates carried other clinically relevant ARGs, such as dfrA17, sul1, sul2, tet(A), aph(6)-Id, aph(3’’)-Ib and aadA5. Sixteen different sequence types (STs) were identified, including ST131 (39.1%), ST38 (10.9%) and ST69 (8.7%). One E. coli isolate belonging to novel ST (ST11874) carried multiple virulence factors including genotoxin, salmochelin, aerobactin and yersiniabactin, suggesting that this isolate has potential to cause health concerns in future. Our study reveals presence of clinically relevant ARGs like blaNDM-6 and tet(X4) in pathogenic strains, which have so far not been reported from the clinics in Norway. Our study may thus, provide a framework for population-based surveillance of antibiotic resistance.en_US
dc.language.isoengen_US
dc.titleResistance profiles and diversity of β-lactamases in Escherichia coli strains isolated from city-scale sewage surveillance in Bergen, Norway mimic clinical prevalenceen_US
dc.typePeer revieweden_US
dc.typeJournal articleen_US
dc.description.versionpublishedVersionen_US
dc.source.pagenumber8en_US
dc.source.journalEcotoxicology and Environmental Safetyen_US
dc.identifier.doi10.1016/j.ecoenv.2021.112788
dc.identifier.cristin1941548
dc.relation.projectNorges forskningsråd: 315266en_US
dc.relation.projectHavforskningsinstituttet: 15495en_US
cristin.ispublishedtrue
cristin.fulltextoriginal
cristin.qualitycode2


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