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dc.contributor.authorOlsvik, Pål Asgeir
dc.contributor.authorHammer, SK
dc.contributor.authorSanden, Monica
dc.contributor.authorSøfteland, Liv Ingeborg Rosvoll
dc.date.accessioned2020-02-06T10:39:33Z
dc.date.available2020-02-06T10:39:33Z
dc.date.created2019-10-31T11:57:13Z
dc.date.issued2019
dc.identifier.citationToxicology in Vitro. 2019, 61 1-13.nb_NO
dc.identifier.issn0887-2333
dc.identifier.urihttp://hdl.handle.net/11250/2639988
dc.description.abstractExposure to contaminants can lead to accumulation of lipids in the liver. This study aimed to examine whether eicosapentaenoic acid (EPA) and arachidonic acid (ARA) supplementation can protect fish cells against the negative impact of chlorpyrifos (CPF). Atlantic salmon hepatocytes were exposed to either 100 μM CPF, 200 μM EPA, 200 μM ARA, or combinations of these for 48 h, and endpoints included lipid droplet formation, gene expression, and global metabolomic analysis. The results showed that polyunsaturated fatty acid (PUFA) supplementation modified the cell lipid composition, reduced uptake of CPF and increased the cellular number and size of lipid droplets. CPF exposure induced the transcription of ppara and fabp3, and reduced the levels of several PUFAs, and lead to accumulation of monoacylglycerols (MAGs) in the cells. Supplementation of EPA or ARA did not prevent CPF-induced accumulation of MAGs and only to a limited degree rescued the response on other lipids. CPF exposure further reduced energy metabolism, a response partly restored by PUFA supplementation. Reduced levels of glutathione indicated oxidative stress; an effect not ameliorated by the PUFAs. Altogether, this study shows that PUFA supplementation only modestly protects Atlantic salmon hepatocytes against the negative impact of CPF.nb_NO
dc.language.isoengnb_NO
dc.titleChlorpyrifos-induced dysfunction of lipid metabolism is not restored by supplementation of polyunsaturated fatty acids EPA and ARA in Atlantic salmon liver cellsnb_NO
dc.typeJournal articlenb_NO
dc.typePeer reviewednb_NO
dc.description.versionpublishedVersionnb_NO
dc.source.pagenumber1-13nb_NO
dc.source.volume61nb_NO
dc.source.journalToxicology in Vitronb_NO
dc.identifier.doi10.1016/j.tiv.2019.104655
dc.identifier.cristin1742798
dc.relation.projectNorges forskningsråd: 200506nb_NO
cristin.unitcode7431,0,0,0
cristin.unitcode7431,33,0,0
cristin.unitcode7431,32,0,0
cristin.unitnameHavforskningsinstituttet
cristin.unitnameFremmed- og smittestoff
cristin.unitnameTrygt fôr
cristin.ispublishedtrue
cristin.fulltextoriginal
cristin.qualitycode1


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