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dc.contributor.authorOlsvik, Pål Asgeir
dc.contributor.authorLie, Kai Kristoffer
dc.contributor.authorMykkeltvedt, Eva
dc.contributor.authorSamuelsen, Ole Bent
dc.contributor.authorPetersen, Kjell
dc.contributor.authorStavrum, Anne-Kristin
dc.contributor.authorLunestad, Bjørn Tore
dc.date.accessioned2020-01-22T08:41:29Z
dc.date.available2020-01-22T08:41:29Z
dc.date.created2019-10-17T14:31:30Z
dc.date.issued2008
dc.identifier.citationBMC Pharmacology. 2008, 8 .nb_NO
dc.identifier.issn1471-2210
dc.identifier.urihttp://hdl.handle.net/11250/2637393
dc.description.abstractBackground Emamectin benzoate (EB) is a dominating pharmaceutical drug used for the treatment and control of infections by sea lice (Lepeophtheirus salmonis) on Atlantic salmon (Salmo salar L). Fish with an initial mean weight of 132 g were experimentally medicated by a standard seven-day EB treatment, and the concentrations of drug in liver, muscle and skin were examined. To investigate how EB affects Atlantic salmon transcription in liver, tissues were assessed by microarray and qPCR at 7, 14 and 35 days after the initiation of medication. Results The pharmacokinetic examination revealed highest EB concentrations in all three tissues at day 14, seven days after the end of the medication period. Only modest effects were seen on the transcriptional levels in liver, with small fold-change alterations in transcription throughout the experimental period. Gene set enrichment analysis (GSEA) indicated that EB treatment induced oxidative stress at day 7 and inflammation at day 14. The qPCR examinations showed that medication by EB significantly increased the transcription of both HSP70 and glutathione-S-transferase (GST) in liver during a period of 35 days, compared to un-treated fish, possibly via activation of enzymes involved in phase II conjugation of metabolism in the liver. Conclusion This study has shown that a standard seven-day EB treatment has only a modest effect on the transcription of genes in liver of Atlantic salmon. Based on GSEA, the medication seems to have produced a temporary oxidative stress response that might have affected protein stability and folding, followed by a secondary inflammatory response.nb_NO
dc.language.isoengnb_NO
dc.titlePharmacokinetics and transcriptional effects of the anti-salmon lice drug emamectin benzoate in Atlantic salmon (Salmo salar L.)nb_NO
dc.typeJournal articlenb_NO
dc.description.versionpublishedVersionnb_NO
dc.source.pagenumber14nb_NO
dc.source.volume8nb_NO
dc.source.journalBMC Pharmacologynb_NO
dc.identifier.doi10.1186/1471-2210-8-16
dc.identifier.cristin1738038
cristin.unitcode7431,32,0,0
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cristin.unitcode7431,33,0,0
cristin.unitnameTrygt fôr
cristin.unitnameHavforskningsinstituttet
cristin.unitnameSykdom og smittespredning
cristin.unitnameFremmed- og smittestoff
cristin.ispublishedtrue
cristin.fulltextoriginal
cristin.qualitycode0


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