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dc.contributor.authorOlsvik, Pål A.
dc.contributor.authorLie, Kai Kristoffer
dc.contributor.authorOlderbakk Jordal, Anne-Lise
dc.contributor.authorNilsen, Tom Ole
dc.contributor.authorHordvik, Ivar
dc.date.accessioned2020-01-14T11:51:07Z
dc.date.available2020-01-14T11:51:07Z
dc.date.created2008-02-01T17:34:35Z
dc.date.issued2005
dc.identifier.citationBMC Molecular Biology. 2005, 6 (21), 1-9.nb_NO
dc.identifier.issn1471-2199
dc.identifier.urihttp://hdl.handle.net/11250/2636174
dc.description.abstractBackground Salmonid fishes are among the most widely studied model fish species but reports on systematic evaluation of reference genes in qRT-PCR studies is lacking. Results The stability of six potential reference genes was examined in eight tissues of Atlantic salmon (Salmo salar), to determine the most suitable genes to be used in quantitative real-time RT-PCR analyses. The relative transcription levels of genes encoding 18S rRNA, S20 ribosomal protein, β-actin, glyceraldehyde-3P-dehydrogenase (GAPDH), and two paralog genes encoding elongation factor 1A (EF1AA and EF1AB) were quantified in gills, liver, head kidney, spleen, thymus, brain, muscle, and posterior intestine in six untreated adult fish, in addition to a group of individuals that went through smoltification. Based on calculations performed with the geNorm VBA applet, which determines the most stable genes from a set of tested genes in a given cDNA sample, the ranking of the examined genes in adult Atlantic salmon was EF1AB>EF1AA>β-actin>18S rRNA>S20>GAPDH. When the same calculations were done on a total of 24 individuals from four stages in the smoltification process (presmolt, smolt, smoltified seawater and desmoltified freshwater), the gene ranking was EF1AB>EF1AA>S20>β-actin>18S rRNA>GAPDH. Conclusion Overall, this work suggests that the EF1AA and EF1AB genes can be useful as reference genes in qRT-PCR examination of gene expression in the Atlantic salmon.nb_NO
dc.language.isoengnb_NO
dc.titleEvaluation of potential reference genes in real-time RT-PCR studies of Atlantic salmonnb_NO
dc.typeJournal articlenb_NO
dc.typePeer reviewednb_NO
dc.description.versionpublishedVersionnb_NO
dc.source.pagenumber1-9nb_NO
dc.source.volume6nb_NO
dc.source.journalBMC Molecular Biologynb_NO
dc.source.issue21nb_NO
dc.identifier.doi10.1186/1471-2199-6-21
dc.identifier.cristin364648
cristin.unitcode7431,32,0,0
cristin.unitcode7431,0,0,0
cristin.unitnameTrygt fôr
cristin.unitnameHavforskningsinstituttet
cristin.ispublishedtrue
cristin.fulltextoriginal
cristin.qualitycode1


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