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dc.contributor.authorWinge, Per
dc.contributor.authorDatsomor, Alex
dc.contributor.authorOlsen, Rolf Erik
dc.contributor.authorJin, Yang
dc.contributor.authorVik, Jon Olav
dc.contributor.authorEdvardsen, Rolf
dc.contributor.authorGrammes, Fabian
dc.contributor.authorWargelius, Anna
dc.contributor.authorLi, Keshuai
dc.date.accessioned2019-06-06T10:32:34Z
dc.date.available2019-06-06T10:32:34Z
dc.date.created2019-06-05T10:40:26Z
dc.date.issued2019
dc.identifier.citationScientific Reports. 2019, 9 .nb_NO
dc.identifier.issn2045-2322
dc.identifier.urihttp://hdl.handle.net/11250/2600120
dc.description.abstractAtlantic salmon can synthesize polyunsaturated fatty acids (PUFAs), such as eicosapentaenoic acid (20:5n-3), arachidonic acid (20:4n-6) and docosahexaenoic acid (22:6n-3) via activities of very long chain fatty acyl elongases (Elovls) and fatty acyl desaturases (Fads), albeit to a limited degree. Understanding molecular mechanisms of PUFA biosynthesis and regulation is a pre-requisite for sustainable use of vegetable oils in aquafeeds as current sources of fish oils are unable to meet increasing demands for omega-3 PUFAs. By generating CRISPR-mediated elovl2 partial knockout (KO), we have shown that elovl2 is crucial for multi-tissue synthesis of 22:6n-3 in vivo and that endogenously synthesized PUFAs are important for transcriptional regulation of lipogenic genes in Atlantic salmon. The elovl2-KOs showed reduced levels of 22:6n-3 and accumulation of 20:5n-3 and docosapentaenoic acid (22:5n-3) in the liver, brain and white muscle, suggesting inhibition of elongation. Additionally, elovl2-KO salmon showed accumulation of 20:4n-6 in brain and white muscle. The impaired synthesis of 22:6n-3 induced hepatic expression of sterol regulatory element binding protein-1 (srebp-1), fatty acid synthase-b, Δ6fad-a, Δ5fad and elovl5. Our study demonstrates key roles of elovl2 at two penultimate steps of PUFA synthesis in vivo and suggests Srebp-1 as a main regulator of endogenous PUFA synthesis in Atlantic salmon.nb_NO
dc.language.isoengnb_NO
dc.titleCRISPR/Cas9-mediated ablation of elovl2 in Atlantic salmon (Salmo salar L.) inhibits elongation of polyunsaturated fatty acids and induces Srebp-1 and target genesnb_NO
dc.typeJournal articlenb_NO
dc.typePeer reviewednb_NO
dc.description.versionpublishedVersionnb_NO
dc.source.pagenumber13nb_NO
dc.source.volume9nb_NO
dc.source.journalScientific Reportsnb_NO
dc.identifier.doi10.1038/s41598-019-43862-8
dc.identifier.cristin1702874
cristin.unitcode7431,24,0,0
cristin.unitnameReproduksjon og utvikl.biologi
cristin.ispublishedtrue
cristin.fulltextoriginal
cristin.qualitycode1


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