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dc.contributor.authorØvergård, Aina-Cathrine
dc.contributor.authorNepstad, Ina
dc.contributor.authorNerland, Audun H.
dc.contributor.authorPatel, Sonal
dc.date.accessioned2019-01-21T11:46:01Z
dc.date.available2019-01-21T11:46:01Z
dc.date.created2012-02-01T14:16:42Z
dc.date.issued2012
dc.identifier.citationMolecular Biology Reports. 2012, 39 (3), 2201-2213.nb_NO
dc.identifier.issn0301-4851
dc.identifier.urihttp://hdl.handle.net/11250/2581491
dc.description.abstractGenes encoding the five Atlantic halibut (Hippoglossus hippoglossus L.) cytokines; interleukin (IL)-1β, IL-6, IL-11b, IL-12βc, and interferon (IFN) γ, were cloned and characterised at a molecular level. The genomic organisation of the halibut cytokine genes was similar to that seen in mammals and/or other fish species. Several mRNA instability motifs were found within the 3′-untranslated region (UTR) of all cytokine cDNA sequences. The putative cytokine protein sequences showed a low sequence identity with the corresponding homologues in mammals, avian and other fish species. Nevertheless, important structural features were presumably conserved such as the presence, or absence in the case of IL-1β, of a signal peptide, secondary structure and family signature motifs. The relative expression pattern of the cytokine genes was analyzed in several halibut organs, revealing a constitutive expression in both lymphoid and non-lymphoid organs. Interestingly, the gills showed a relatively high expression of IL-1β, IL-12βc and IFNγ. The real time RT-PCR data also showed that the mRNA level of IL-1β, IL-6, IL-12βc and IFNγ was high in the thymus, while IL-11b was relatively highly expressed in the posterior kidney and posterior gut. Moreover, the halibut brain showed a relatively high level of IL-6 transcripts. Anterior kidney leucocytes in vitro stimulated with imiquimod showed a significant increase in mRNA level of the five halibut cytokine genes. The sequence and characterisation data presented here will be useful for further investigation of both innate and adaptive immune responses in halibut, and be helpful in the design of vaccines for the control of various infectious diseases.nb_NO
dc.language.isoengnb_NO
dc.titleCharacterisation and expression analysis of the Atlantic halibut (Hippoglossus hippoglosssus L.) cytokines: IL-1β, IL-6, IL-11, IL-12β and IFNγnb_NO
dc.title.alternativeCharacterisation and expression analysis of the Atlantic halibut (Hippoglossus hippoglosssus L.) cytokines: IL-1β, IL-6, IL-11, IL-12β and IFNγnb_NO
dc.typeJournal articlenb_NO
dc.typePeer reviewednb_NO
dc.description.versionpublishedVersionnb_NO
dc.subject.nsiVDP::Molekylærbiologi: 473nb_NO
dc.subject.nsiVDP::Molecular biology: 473nb_NO
dc.source.pagenumber2201-2213nb_NO
dc.source.volume39nb_NO
dc.source.journalMolecular Biology Reportsnb_NO
dc.source.issue3nb_NO
dc.identifier.doi10.1007/s11033-011-0969-x
dc.identifier.cristin903145
cristin.unitcode7431,17,0,0
cristin.unitnameSykdom og smittespredning
cristin.ispublishedtrue
cristin.fulltextoriginal
cristin.qualitycode1


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