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dc.contributor.authorOlsen, Anne Berit
dc.contributor.authorNilsen, Helene
dc.contributor.authorSandlund, Nina
dc.contributor.authorMikkelsen, Helene
dc.contributor.authorSørum, H.
dc.contributor.authorColquhoun, D.J.
dc.date.accessioned2011-11-03T13:19:42Z
dc.date.issued2011-05-09
dc.identifier.urihttp://hdl.handle.net/11250/117158
dc.description.abstractColdwater-associated ulcers, i.e. winter ulcers, in seawater-reared Atlantic salmon Salmo salar L. have been reported in Norway since the late 1980s, and Moritella viscosa has been established as an important factor in the pathogenesis of this condition. As routine histopathological examination of winter ulcer cases in our laboratory revealed frequent presence in ulcers of long, slender rods clearly different from M. viscosa, a closer study focusing on these bacteria was conducted. Field cases of winter ulcers during 2 sampling periods, 1996 and 2004–2005, were investigated and long, slender rods were observed by histopathological examination in 70 and 62.5% of the ulcers examined, respectively, whereas cultivation on marine agar resulted in the isolation of yellowpigmented colonies with long rods from 3 and 13% of the ulcers only. The isolates could be separated into 2 groups, both identified as belonging to the genus Tenacibaculum based on phenotypic characterization and 16S rRNA sequencing. Bath challenge for 7 h confirmed the ability of Group 1 bacterium to produce skin and cornea ulcers. In fish already suffering from M. viscosa-induced ulcers, co-infection with the Group 1 bacterium was established within 1 h. Ulcers from field cases of winter ulcers and from the transmission experiments tested positive by immunohistochemistry with polyclonal antiserum against the Group 1 bacterium but not the Group 2 bacterium. Our results strongly indicate the importance of the Group 1 bacterium in the pathogenesis of winter ulcers in Norway. The bacterium is difficult to isolate and is therefore likely to be underdiagnosed based on cultivation only.no_NO
dc.language.isoengno_NO
dc.publisherInter-Researchno_NO
dc.subjecthalibutno_NO
dc.subjectatlantic salmonno_NO
dc.subjectcodno_NO
dc.subjecttorsk
dc.subjectatlantisk laks
dc.subjectkveite
dc.titleTenacibaculum sp. associated with winter ulcers in sea-reared Atlantic salmon Salmo salarno_NO
dc.typeJournal articleno_NO
dc.typePeer reviewedno_NO
dc.subject.nsiVDP::Agriculture and fishery disciplines: 900::Fisheries science: 920::Fish health: 923no_NO
dc.subject.nsiVDP::Agriculture and fishery disciplines: 900::Fisheries science: 920::Aquaculture: 922no_NO
dc.description.embargo2016-05-09
dc.source.pagenumber189-199no_NO
dc.source.volume94no_NO
dc.source.journalDiseases of Aquatic Organismsno_NO
dc.source.issue3no_NO
dc.identifier.doihttp://dx.doi.org/10.3354/dao02324


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