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dc.contributor.authorMinghetti, M.
dc.contributor.authorOlsen, Rolf Erik
dc.contributor.authorTocher, D.R.
dc.date.accessioned2011-09-21T13:26:55Z
dc.date.issued2010-10
dc.identifier.issn1353-5773
dc.identifier.issn1365-2095
dc.identifier.urihttp://hdl.handle.net/11250/117019
dc.description.abstractPrevious studies had shown that wax ester-rich lipid extracted from calanoid copepods could be a useful alternative to fish oil as a provider of long-chain n-3 polyunsaturated fatty acids in diets for use in salmon aquaculture. Effective utilization of wax ester requires digestion and metabolism in the intestine with the fatty alcohol component being oxidized to fatty acid in intestinal cells through the combined activities of alcohol dehydrogenase (ADH) and aldehyde dehydrogenase (ALDH). We studied wax ester utilization in Atlantic salmon using a candidate gene approach, focusing on ADH and ALDH as sequence information was available for these genes, including fish sequences, facilitating isolation of the cDNAs. Here, we report on the isolation and cloning of full-length cDNAs for ADH3 and ALDH3a2 genes from salmon intestinal tissue. Functional characterization by heterologous expression in the yeast, Saccharomyces cerevisiae, showed the products of these cDNAs had long-chain ADH and ALDH enzyme activities. Thus, ADH3 was capable of oxidizing long-chain fatty alcohol, and ALDH3a2 was capable of oxidizing long-chain fatty aldehyde to the corresponding fatty acid. The genes were highly expressed in intestinal tissue, particularly pyloric caeca, but their expression was not increased in salmon fed dietary copepod oil in comparison to fish fed fish oil.en_US
dc.language.isoengen_US
dc.publisherBlackwellen_US
dc.subjectnutritionen_US
dc.subjecternæringen_US
dc.subjectcopepodaen_US
dc.subjectcopepodsen_US
dc.subjectfatty acidsen_US
dc.subjectfettsyreren_US
dc.subjectsalmon farmingen_US
dc.subjectlakseoppdretten_US
dc.titleMolecular cloning, characterization and nutritional regulation of key enzymes required for the effective utilization of marine wax esters by Atlantic salmon (Salmo salar L.)en_US
dc.typeJournal articleen_US
dc.typePeer revieweden_US
dc.subject.nsiVDP::Agriculture and fishery disciplines: 900::Fisheries science: 920::Aquaculture: 922en_US
dc.description.embargo10000-01-01
dc.source.pagenumber483-495en_US
dc.source.volume16en_US
dc.source.journalAquaculture Nutrition
dc.source.issue5en_US
dc.identifier.doihttp://dx.doi.org/10.1111/j.1365-2095.2009.00687.x


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