Development of eleven microsatellite loci in the deep-sea black scabbardfish (Aphanopus carbo)

Abstract

We developed 11 microsatellite loci primers in the bathypelagic black scabbardfish (Aphanopus carbo), a novelty for deep-sea fishes. All markers were obtained from partial genomic DNA libraries enriched for tetranucleotide repeats and characterized in 50 unrelated individuals from one putative population. The number of alleles ranged from 5 to 40, with an average of 19.3 per locus, and the observed heterozygosity ranged from 0.387 to 0.961 (average 0.749). Cross-amplification in another closely related commercially exploited deep-sea species intermediate scabbardfish (Aphanopus intermedius) resolved 11 polymorphic loci.