Development of twelve microsatellite loci in the corkwing wrasse (Symphodus melops)

Abstract

We developed 12 microsatellite loci primers in 8 the corkwing wrasse (Symphodus melops). All markers 9 were obtained from partial genomic DNA libraries enri-10 ched for tetranucleotide repeats and characterized in 32 11 unrelated individuals from one putative population. The 12 number of alleles ranged from 5 to 18, with an average of 13 8.6 per locus, and the observed heterozygosity ranged from 14 0.464 to 0.969 (average 0.697). Cross-amplification in two 15 closely related commercially exploited species, the ballian 16 wrasse (Labrus bergylta) and the goldsinny wrasse (Cten- 17 olabrus rupestris), successfully resolved four loci of which 18 two were polymorphic and two where monomorphic.