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dc.contributor.authorStefanni, Sergio
dc.contributor.authorBettencourt, Raul
dc.contributor.authorKnutsen, Halvor
dc.contributor.authorMenezes, Gui
dc.date.accessioned2010-12-13T13:13:21Z
dc.date.available2010-12-13T13:13:21Z
dc.date.issued2009-03
dc.identifier.issn1755-0998
dc.identifier.issn1755-098X
dc.identifier.urihttp://hdl.handle.net/11250/108444
dc.descriptionJournal homepage: http://onlinelibrary.wiley.com/journal/10.1111/(ISSN)1755-0998en_US
dc.description.abstractThe present investigation provides an efficient diagnostic method based on polymerase chain reaction–restriction fragment length polymorphism (PCR–RFLP) analysis to discriminate between two cryptic species of scabbardfish, Aphanopus carbo and A. intermedius, with commercial relevance in several European fish markets. Two DNA fragments from the mtDNA, including control region and partial cytochrome oxidase subunit I genes of about 1100 bp and 700 bp, respectively, were isolated by PCR amplification. Digestion of the amplicon including the control region with HaeII and the amplicon including the COI gene with Sau3AI restriction enzymes allowed an unequivocal discrimination between the two scabbardfish species. This PCR–RFLP method allowed a clear and rapid discrimination of the trichiurid species studied.en_US
dc.language.isoengen_US
dc.publisherBlackwellen_US
dc.subjectblack scabbardfishen_US
dc.subjectdolkfisken_US
dc.subjectAtlantic Seaen_US
dc.subjectAtlanterhaveten_US
dc.titleRapid polymerase chain reaction–restriction fragment length polymorphism method for discrimination of the two Atlantic cryptic deep-sea species of scabbardfishen_US
dc.typeJournal articleen_US
dc.typePeer revieweden_US
dc.subject.nsiVDP::Agriculture and fishery disciplines: 900::Fisheries science: 920::Resource biology: 921en_US
dc.source.pagenumber528-530en_US
dc.source.volume9
dc.source.journalMolecular Ecology Resources
dc.source.issue2
dc.identifier.doihttp://dx.doi.org/10.1111/j.1755-0998.2008.02470.x


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