• norsk
    • English
  • norsk 
    • norsk
    • English
  • Logg inn
Vis innførsel 
  •   Hjem
  • Havforskningsinstituttet
  • Published externally
  • Articles
  • Vis innførsel
  •   Hjem
  • Havforskningsinstituttet
  • Published externally
  • Articles
  • Vis innførsel
JavaScript is disabled for your browser. Some features of this site may not work without it.

Coho salmon (Oncorhynchus kisutch) transgenic for a growth hormone gene construct exhibit increased rates of muscle hyperplasia and detectable levels of differential gene expression

Hill, James A.; Kiessling, Anders; Devlin, Robert H.
Journal article, Peer reviewed
Thumbnail
Åpne
2000_939.pdf (743.5Kb)
Permanent lenke
http://hdl.handle.net/11250/108182
Utgivelsesdato
2000
Metadata
Vis full innførsel
Samlinger
  • Articles [2388]
Originalversjon
http://dx.doi.org/10.1139/f00-015
Sammendrag
Transgenic coho salmon (Oncorhynchus kisutch) containing a growth hormone gene construct were compared

with nontransgenic coho salmon in terms of gross anatomy, muscle cellularity, muscle enzyme activity, and differential

gene expression. Transgenic fish were found to have significantly higher numbers of small-diameter muscle fibres in

both the dorsal and lateral region of the somitic muscle, suggesting that they grow by greater rates of hyperplasia relative

to slower growing nontransgenic fish. Higher levels of activity were found for phosphofructokinase and cytochrome

oxidase in white muscle of the transgenic fish. This difference indicates a higher glycolytic and aerobic requirement in

the muscle of transgenic fish. Subtractive hybridisation of muscle RNA of transgenic fish from control fish provided a

library of cDNAs whose expression is upregulated in the transgenic fish. This library contains genes that may be involved

in, or related to, both high growth rates and muscle hyperplasia. We have sequenced a number of fragments and

have found a preponderance of myosin light chain 2 mRNAs, consistent with a putative high level of expression in the

early stages of muscle fibre construction.
Tidsskrift
Canadian Journal of Fisheries and Aquatic Sciences

Kontakt oss | Gi tilbakemelding

Personvernerklæring
DSpace software copyright © 2002-2019  DuraSpace

Levert av  Unit
 

 

Bla i

Hele arkivetDelarkiv og samlingerUtgivelsesdatoForfattereTitlerEmneordDokumenttyperTidsskrifterDenne samlingenUtgivelsesdatoForfattereTitlerEmneordDokumenttyperTidsskrifter

Min side

Logg inn

Statistikk

Besøksstatistikk

Kontakt oss | Gi tilbakemelding

Personvernerklæring
DSpace software copyright © 2002-2019  DuraSpace

Levert av  Unit